Raleigh, NC | Pineville, NC | Catawba, NC
Also known as: urgurljamie69
MS Biomanufacturing Student graduating December with experience in viral vaccine downstream processing and analytical characterization of both egg and cell-based influenza vaccine systems. Hands-on graduate coursework includes validation, process development, and scale-up of both upstream and downstream unit operations. Most recent experience as engineering intern within Manufacturing Technical Sciences (MTS) supporting existing process understanding and process transfer to new US-based cGMP manufacturing site. Additional professional and research work includes years analytical lab experience, teaching, team leading, and process development Masters thesis project. Upstream -Development of yeast (Pichia pastoris) fermentation process -Recombinant E. coli fermentations in shake flask, bench-scale, L, and L reactors -Egg-based vaccine processing -Tissue culture Downstream -Viral inactivation -Centrifugation -Homogenization -Chromatography (Hydrophobic Interaction, Ion Exchange, Affinity, Size Exclusion, HPLC, AKTA) -Tangential Flow Filtration (ConcentrationDiafiltration) -Sterile filtration Analytical and Laboratory -Cloning and Gene Expression -SDS-PAGE -Biochemical Assays -Column chromatography -HPLC -MALDI-TOF -Capillary Electrophoresis Software and Technology MFCSwin, LabView, LabX, LIMS, WinStat, Microsoft Office (Excel, Publisher, Powerpoint, Word, Outlook), SmartDraw, OmniGraffle, MiniTab
Jamie Sigmon lives in Pineville, North Carolina. He has also lived in Conover, North Carolina. He is an alumnus of the Central Piedmont Community College, University Of North Carolina At Charlotte, Catawba Valley Community College and Independence High School. Companies in which he has a work history include Service Merchandise and Crg. Jobs he has held in the past include Contractor.
Also known as: jamiesigmon
Jamie Sigmon lives in Conover, North Carolina. He is an alumnus of the Central Piedmont Community College, University Of North Carolina At Charlotte, Catawba Valley Community College and Independence High School. Companies in which he has a work history include Service Merchandise and Crg. Jobs he has held in the past include Contractor. Rap, Metal, Punk, Rock, Hip Hop and R&b are his favorite bands. Jamie loves American Idol, Csi, Bones, Ncis, House and Discovery Channel. Blades Of Glory and Close To Home are his favorite movies. His favorite sport is Parasailing.
Jamie Sigmon lives in Hickory, NC, but has also spent time in Claremont, NC. She is a 2005 graduate of Bunker Hill High School and she is employed by Nursing. Jamie a big fan of Rap and Rock.
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Jamie Simone is an American voice director and producer, best known for directing Naruto, Sailor Moon and Tiger & Bunny. He is the owner of the recording studio Studiopolis. In 2012, he was nominated for a Daytime Emmy Award for "Outstanding Directing in an Animated Program" for Transformers: Prime, which he took over from Susan Blu.. You can find more here.
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