Also known as: ADRPHD
My research focuses on human tumor metabolism with the dual goals of therapeutic discovery and technology development. We have developed stable isotope-based metabolic tracer methods which allow us to determine metabolic fluxes in human cells. By supplying C-labled metabolic substrates (such as glucose, glutamine, and lactate) to living cells, we are able to then analyze isotopomer patterns of key metabolites by mass spectrometry and NMR. This data yields information on both pathway activities and metabolite pool sizes. In particular, we are able to determine activity rates of glycolysis, the pentose phosphate pathway, glutaminolysis, the TCA cycle, fatty acid biosynthesis and oxidation, and the biosynthesis of most non-essential amino acids. In addition, we have the ability to perform a wide range of complementary assays, such as large scale unbiased metabolite profiling by LCMS, rapid substrate profiling by YSI analyzer, bioenergetic analysis (NAD(P)H and ATPADPAMP) by HPLC and respiratory capacity by Seahorse XF. The understanding gained from these experiments allows us to identify potential anti-tumor targets that we then confirm using a combination of molecular and chemical biology approaches. Overall, this research leads to novel insights and reveals connections between oncogenes, signaling pathways and the unique metabolic program of tumor cells.
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Adam Scott Richardson is a former Australian rules footballer who played for West Adelaide in the South Australian National Football League (SANFL) and Adelaide in the Australian Football League (AFL).. You can find more here.
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